What makes a biological clock efficient? - Microsoft


What makes a biological clock efficient? Attila Csikász-Nagy1,2, Neil Dalchau3 1

Randall Division of Cell and Molecular Biophysics and Institute for Mathematical and Molecular Biomedicine, King's College London, London SE1 1UL, United Kingdom


Department of Computational Biology, Research and Innovation Center, Fondazione Edmund Mach, San Michele all'Adige 38010, Italy


Microsoft Research, Cambridge CB1 2FB, United Kingdom Abstract

Biological clocks regulate the proper periodicity of several processes at the cellular and organismal level. The cell cycle and circadian rhythm are the best characterized among these but several other biological clocks function in cells at widely variable periodicity. The underlying molecular networks are controlled by delayed negative feedbacks, but the role of positive feedbacks and substrate-depletion has been also proposed to play crucial roles in the regulation of these processes. Here we will investigate which features of biological clocks might be important for their efficient timekeeping.

Evolution of biological clocks The ability of organisms to temporally co-ordinate their physiology is evolutionarily advantageous, and is therefore ubiquitous in nature. Organisms have evolved numerous so-called biological clocks to optimise their fitness, by co-ordinating their physiology with the availability of resources. Simple experiments monitoring the growth of bacteria against varying nutrient availability illustrate an enormous flexibility in deciding how frequently cells choose to divide. Yet, the developmental programs that lead to the replication of entire organisms (mammals) are relatively inflexible. The cell cycle, which culminates in cell division, is controlled by regulatory networks that have numerous conserved features and components across the eukaryotic kingdom (Harashima et al., 2013). Circadian clocks allow organisms to co-ordinate their physiology with the external time of day, enabling anticipation of changes in temperature, light availability, predator activity, etc. In contrast to the cell cycle, circadian clocks have evolved multiple times and have many different features (though some shared components) across the eukaryotic kingdom (Dalchau and Webb, 2011). The overall structure of circadian networks involves input pathways, a core oscillator, and output pathways (Dunlap, 1999). The core oscillator comprises multiple feedback loops that sustain circadian rhythms with a period of approximately 24 h. Input pathways enable the 1

oscillator to maintain synchrony with external time, while output pathways provide the biochemical means of the oscillator to regulate downstream physiology, including gene expression, metabolism and signalling.

Synthesizing biological clocks In recent years, there has been a large rise in the number of attempts to engineer biological systems. The field of synthetic biology seeks to improve understanding of biological functions by attempting to re-create specific systems and their behaviours, using existing cells and their housekeeping components (RNA polymerase, ribosomes, proteasomes, etc.) as a chassis. The creation of biological devices is beginning to open new opportunities in industry, for example using bacteria to produce biofuels and medicines. A seminal work in this field was the construction of a biological clock, termed the repressilator, in which three transcriptional repressors were taken from non-oscillatory networks and inserted into Escherichia coli on a plasmid, but arranged as a cycle of repression (Elowitz and Leibler, 2000). Briefly, TetR was placed under the control of a LacI-repressible promoter, LacI was placed under the control of a CI-repressible promoter, and CI under the control of a TetRrepressible promoter. It was demonstrated that oscillations in the abundance of the constituent proteins could be generated when the strengths of the interactions between the repressor proteins and their cognate DNA-binding domains were tuned to appropriate levels.

Figure 1. Deterministic simulation of ring oscillators. Sequential inhibition of transcriptional repressors around a single feedback loop produces oscillations. a. Simulation of the repressilator (3-component ring oscillator) model in Elowitz & Leibler. b,c. Extending the model to 5 and 11 components also yields oscillations in protein copy number. Protein 1 is plotted with a thickened black line to emphasize differences in oscillation waveform between different degree ring oscillators.

The repressilator network is an example of a 3-stage ring oscillator. Ring oscillators are often used in electrical engineering for generating oscillations. However, only rings with an odd number of components can give oscillatory dynamics ((Sprinzak and Elowitz, 2005); examples in Figure 1). This is because each regulator inverts the gradient of the following regulator, which for an even number of components would result in an equilibrium ON-OFF-ON-OFF-…-ON-OFF. Using an odd number of


components breaks this pattern, and can yield oscillations. Increasing the length of the feedback loop with additional components leads to more square-like waveforms (Figure 1). It has recently been established that a repressilator motif also exists in nature, lying at the heart of the circadian clock in plants (Pokhilko et al., 2012) and in the core of transcriptional regulation of the cell cycle (Sriram et al., 2007). Oscillators have also been created in mammals (Tigges et al., 2009) as well as in cell-free conditions, mixing chemical compounds in such a way as to recapitulate interaction networks that can exhibit oscillatory behaviour, for instance using negative feedback. In various works dating back to the 1950s, the famous BelousovZhabotinsky reaction was shown to both oscillate in time and propagate over excitable media (Field et al., 1972). More recently, the construction of chemical oscillators made from DNA has been demonstrated, inspired by predator-prey (PP) cycles (Fujii and Rondelez, 2012). A system of 3 reactions is sufficient to generate PP cycles: i. an autocatalytic growth of the prey species, ii. an autocatalytic predation of the prey, and iii. decay of the predator species. The DNA-based PP network relies on DNA polymerization-depolymerization reactions to recapitulate these reactions, and is capable of sustaining many (>20) cycles before eventual depletion of necessary cofactors.

Mathematical analysis of biological clock architectures There is a long history on the mathematical analysis of biological clocks (Goldbeter, 1997, 2002), still it is not fully understood what makes such a periodic system efficient. Biological clocks can run with a period of seconds (neural, cardiac, calcium rhythms) to months and years (ovarian, annual and ecological rhythms) and are regulated by delayed negative feedback loops that cause oscillations in the activities of system components (Goldbeter, 2008). Direct negative feedback loops lead to stabilization of steady states but delay in the loop and non-linearity in the interactions can induce oscillations (Goodwin, 1965; Griffith, 1968). This generic rule that delayed negative feedback loops form the basis of biological oscillations is now very well established for many biological clocks (Fig. 2A). The daily rhythms of the circadian clock might be the best example, where it was established that the existence of a direct time delay caused by a transcriptional-translational loop is driving the periodic appearance of a transcriptional repressor (Dunlap, 1999). Interestingly it was recently revealed that even in the absence of the delay caused by transcription-translation the circadian clock is robustly ticking (Nakajima et al., 2005; O’Neill et al., 2011). Later it was proposed that a positive feedback loop might play a crucial role in the control of this reduced system (Mehra et al., 2006). Indeed the importance of positive feedback loops in the robustness of circadian clock regulation was proposed at other places as well (Tyson et al., 1999; BeckerWeimann et al., 2004; Hong et al., 2009). These led to the conclusion that the circadian clock is controlled by interactions of positive and negative feedback loops.


Another highly investigated biological clock is driven by the cell cycle regulatory network. The controlled timing of DNA replication and cell division is determined by this clock and again earliest models considered a delayed negative feedback loop to drive this system (Goldbeter, 1991) and later results revealed the importance of positive feedback loops as well (Pomerening et al., 2005; Tsai et al., 2014). Thus it is a reoccurring pattern that crucial biological clocks are regulated by interlinked positive and negative feedback loops (Tsai et al., 2008; Ferrell Jr et al., 2011).


Y* S

Z* A




Figure 2. Feedback loops leading to oscillations. A, negative feedback loop, where protein X activates Y, which activates Z which is eventually inducing the degradation of X. B, Substrate-depletion, where a substrate S is produced and piling up in this form until the product P cannot turn on its autocatalytic loop converting most S into P. As P is less stable than S, the system runs out of both S and P, thus S will pile up again and oscillations emerge.

In the case of glycolytic oscillations of the metabolic system it was proposed very early that a positive feedback loop has a crucial role in controlling this biological clock and the oscillations appear as a result of the depletion of the substrate of an autocatalytic process (Higgins, 1964; Sel'kov, 1968). In this system a stable substrate is produced and converted into an unstable product in an autocatalytic manner (Fig. 2B) leading to oscillations where S is slowly increasing until P reaches a threshold and quickly converts all S into P (Fig. 3). The requirements for this system to oscillate are: i, non-linear autocatalysis on the S P transition, ii, a background S P conversion independent of P to allow P reaching the threshold and iii, removal rate of P has to be much higher than that of S. Note that this system shows high resemblance to the above mentioned predator-prey cycles. In both cases the pile up of one species is followed by the conversion of this species to another species by an autocatalytic step and eventual removal of the second species. Interestingly one of the earliest cell cycle models was also working as a substratedepletion oscillator (Tyson, 1991) and since then it was further established that the kinetics of the substrate-depletion model resemble that of the negative feedback with positive feedback model (Fall et al., 2002). Indeed one can see the delayed negative feedback in the substrate-depletion model as P removes its activator S (by converting it to P). Thus we could state again that interlocked positive and negative feedbacks regulate glycolytic oscillations. It is also important to mention that the substratedepletion mechanism that leads to oscillations in time can drive spatial biological clocks such as pattern formation and emergence of travelling waves (Meinhardt, 1982).


Figure 3. Deterministic simulation of a substrate-depletion oscillator. The substrate S is produced and first slowly converted into P. When P reaches a threshold it converts all S to P, which gets quickly destroyed. Leading to a bursting-like pattern in P oscillations.

Efficiency of biological clocks Going back to the original question in the title: what makes biological clocks efficient? In fact, how to measure the efficiency of biological clocks? The robustness of the periodicity of biological clocks were investigated in the context of the circadian rhythm (Barkai and Leibler, 2000; Gonze et al., 2002) and the cell cycle (Steuer, 2004; Mura and Csikász-Nagy, 2008). Both were found to be quite robust to parameter perturbations and also to intrinsic noise resulting from the low molecular numbers present in the system. So far we have seen many parallels between the circadian clock and cell cycle regulatory systems. There is one major point where they differ. The period of the circadian clock is quite insensitive for temperature changes whereas the cell cycle time can be greatly influenced by alterations in temperature (Klevecz and King, 1982). This result might suggest that the circadian rhythm regulatory network is a more efficient time keeper, while the cell cycle regulatory systems is more efficient in adjusting its period to adapt to environmental changes (Zámborszky et al., 2007; Hong et al., 2014). Changes in temperature affect chemical reactions exponentially, following the Arrhenius equation. How such changes in reaction rates do not influence the period of the circadian clock is a debated question (Tyson et al., 2008). Several models have been worked out to understand what causes the temperature compensation in the circadian clock (Ruoff and Rensing, 1996; Leloup and Goldbeter, 1997; Gould et al., 2006; Hong et al., 2007; François et al., 2012) and some more generic models of temperature compensation in biochemical reaction networks have also been proposed (Ruoff et al., 1997; Hatakeyama and Kaneko, 2012). Recently even a synthetic temperature compensated oscillator was created (Hussain et al., 2014), interestingly containing both a positive and a negative feedback loop. Furthermore non-biological ring oscillators on semiconductors were also designed to be temperature compensated


(Hayashi and Kondoh, 1993). Despite all of these results and theoretical ideas we still lack a coherent generic picture of what makes biological oscillators temperature compensated and in general robust in proper periodicity.

Conclusions Recently it was established by Cardelli and Csikász-Nagy that a class of biological switches follow the dynamical features of an efficient computational algorithm (Cardelli and Csikász-Nagy, 2012). The Approximate Majority (AM) algorithm is used in distributed computing as a population protocol computing the majority of two finite populations by converting the minority population into the majority population (Angluin et al., 2008). It was shown that AM can mimic the dynamics of the cell cycle switch regulatory network that induces the transition between stable cell cycle states. It was also postulated that the cell cycle switch efficiency is maximal only when its dynamics fully captures that of the AM algorithm (Cardelli and Csikász-Nagy, 2012) and later this prediction was experimentally verified (Hara et al., 2012). We have seen that it is very well established that reliable biological time keeping mechanisms are regulated by the interconnection of such switch generating positive feedback loops with oscillation inducing negative feedback loops. The existence of negative feedback is essential and in almost all highly investigated systems the role of the positive feedback is important for the robust behaviour of the biological clock. It was established that the positive feedback module of the cell cycle regulatory network behaves like an efficient algorithm (Cardelli and Csikász-Nagy, 2012). Later, Cardelli (2014) established a theory to identify kinetically identically behaving regulatory networks. A future challenge will be the elucidation of which aspects of real life biological oscillators are important for their proper ticking and how far their kinetics could be associated to a minimalistic oscillator model.

References Angluin, D., Aspnes, J., and Eisenstat, D. (2008). A simple population protocol for fast robust approximate majority. Distributed Computing 21, 87-102. Barkai, N., and Leibler, S. (2000). Biological rhythms: Circadian clocks limited by noise. Nature 403, 267-268. Becker-Weimann, S., Wolf, J., Herzel, H., and Kramer, A. (2004). Modeling Feedback Loops of the Mammalian Circadian Oscillator. Biophysical journal 87, 3023-3034. Cardelli, L. (2014). Morphisms of Reaction Networks that Couple Structure to Function. BMC Systems Biology in press Cardelli, L., and Csikász-Nagy, A. (2012). The cell cycle switch computes approximate majority. Scientific reports 2, 656.


Dalchau, N., and Webb, A.A. (2011). Dalchau, N., and Webb, A.A. (2011). Ticking over. Circadian systems across the kingdoms of life. Biochemist February issue, 1215. Dunlap, J.C. (1999). Molecular bases for circadian clocks. Cell 96, 271-290. Elowitz, M.B., and Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338. Fall, C.P., Marland, E.S., Wagner, J.M., and Tyson, J.J. (2002). Computational cell biology, volume 20 of Interdisciplinary Applied Mathematics: Springer Verlag. Ferrell Jr, J.E., Tsai, T.Y.-C., and Yang, Q. (2011). Modeling the cell cycle: why do certain circuits oscillate? Cell 144, 874-885. Field, R.J., Koros, E., and Noyes, R.M. (1972). Oscillations in chemical systems. II. Thorough analysis of temporal oscillation in the bromate-cerium-malonic acid system. Journal of the American Chemical Society 94, 8649-8664. François, P., Despierre, N., and Siggia, E.D. (2012). Adaptive Temperature Compensation in Circadian Oscillations. PLoS Comput Biol 8, e1002585. Fujii, T., and Rondelez, Y. (2012). Predator–prey molecular ecosystems. Acs Nano 7, 27-34. Goldbeter, A. (1991). A minimal cascade model for the mitotic oscillator involving cyclin and cdc2 kinase. Proceedings of the National Academy of Sciences 88, 91079111. Goldbeter, A. (1997). Biochemical oscillations and cellular rhythms. Biochemical Oscillations and Cellular Rhythms, by Albert Goldbeter, Foreword by MJ Berridge, Cambridge, UK: Cambridge University Press, 1997 1. Goldbeter, A. (2002). Computational approaches to cellular rhythms. Nature 420, 238-245. Goldbeter, A. (2008). Biological rhythms: clocks for all times. Current biology 18, R751-R753. Gonze, D., Halloy, J., and Goldbeter, A. (2002). Robustness of circadian rhythms with respect to molecular noise. Proceedings of the National Academy of Sciences 99, 673-678. Goodwin, B.C. (1965). Oscillatory behavior in enzymatic control processes. Advances in enzyme regulation 3, 425-437. Gould, P.D., Locke, J.C., Larue, C., Southern, M.M., Davis, S.J., Hanano, S., Moyle, R., Milich, R., Putterill, J., and Millar, A.J. (2006). The molecular basis of temperature compensation in the Arabidopsis circadian clock. The Plant Cell Online 18, 1177-1187. Griffith, J. (1968). Mathematics of cellular control processes I. Negative feedback to one gene. Journal of Theoretical Biology 20, 202-208. Hara, M., Abe, Y., Tanaka, T., Yamamoto, T., Okumura, E., and Kishimoto, T. (2012). Greatwall kinase and cyclin B-Cdk1 are both critical constituents of Mphase-promoting factor. Nature communications 3, 1059. Harashima, H., Dissmeyer, N., and Schnittger, A. (2013). Cell cycle control across the eukaryotic kingdom. Trends in cell biology 23, 345-356.


Hatakeyama, T.S., and Kaneko, K. (2012). Generic temperature compensation of biological clocks by autonomous regulation of catalyst concentration. Proceedings of the National Academy of Sciences 109, 8109-8114. Hayashi, I., and Kondoh, H. (1993). Temperature-compensated ring oscillator circuit formed on a semiconductor substrate: US patent 5180995 A. Higgins, J. (1964). A chemical mechanism for oscillation of glycolytic intermediates in yeast cells. Proceedings of the National Academy of Sciences of the United States of America 51, 989. Hong, C.I., Conrad, E.D., and Tyson, J.J. (2007). A proposal for robust temperature compensation of circadian rhythms. Proceedings of the National Academy of Sciences 104, 1195-1200. Hong, C.I., Zámborszky, J., Baek, M., Labiscsak, L., Ju, K., Lee, H., Larrondo, L.F., Goity, A., Chong, H.S., Belden, W.J., and Csikasz-Nagy, A. (2014). Circadian rhythms synchronize mitosis in Neurospora crassa. Proceedings of the National Academy of Sciences 111, 1397-1402. Hong, C.I., Zámborszky, J., and Csikász-Nagy, A. (2009). Minimum criteria for DNA damage-induced phase advances in circadian rhythms. PLoS computational biology 5, e1000384. Hussain, F., Gupta, C., Hirning, A.J., Ott, W., Matthews, K.S., Josić, K., and Bennett, M.R. (2014). Engineered temperature compensation in a synthetic genetic clock. Proceedings of the National Academy of Sciences 111, 972-977. Klevecz, R.R., and King, G.A. (1982). Temperature compensation in the mammalian cell cycle. Experimental cell research 140, 307-313. Leloup, J.-C., and Goldbeter, A. (1997). Temperature compensation of circadian rhythms: control of the period in a model for circadian oscillations of the PER protein in Drosophila. Chronobiology international 14, 511-520. Mehra, A., Hong, C.I., Shi, M., Loros, J.J., Dunlap, J.C., and Ruoff, P. (2006). Circadian rhythmicity by autocatalysis. PLoS computational biology 2, e96. Meinhardt, H. (1982). Models of biological pattern formation. Academic Press London. Mura, I., and Csikász-Nagy, A. (2008). Stochastic Petri Net extension of a yeast cell cycle model. Journal of Theoretical Biology 254, 850-860. Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., Oyama, T., and Kondo, T. (2005). Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. Science 308, 414-415. O’Neill, J.S., Van Ooijen, G., Dixon, L.E., Troein, C., Corellou, F., Bouget, F.-Y., Reddy, A.B., and Millar, A.J. (2011). Circadian rhythms persist without transcription in a eukaryote. Nature 469, 554-558. Pokhilko, A., Fernández, A.P., Edwards, K.D., Southern, M.M., Halliday, K.J., and Millar, A.J. (2012). The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops. Molecular systems biology 8. Pomerening, J.R., Kim, S.Y., and Ferrell Jr, J.E. (2005). Systems-level dissection of the cell-cycle oscillator: bypassing positive feedback produces damped oscillations. Cell 122, 565-578.


Ruoff, P., and Rensing, L. (1996). The temperature-compensated Goodwin model simulates many circadian clock properties. Journal of Theoretical Biology 179, 275285. Ruoff, P., Rensing, L., Kommedal, R., and Mohsenzadeh, S. (1997). Modeling temperature compensation in chemical and biological oscillators. Chronobiology international 14, 499-510. Sel'kov, E. (1968). Self-Oscillations in Glycolysis. European Journal of Biochemistry 4, 79-86. Sprinzak, D., and Elowitz, M.B. (2005). Reconstruction of genetic circuits. Nature 438, 443-448. Sriram, K., Bernot, G., and Kepes, F. (2007). A minimal mathematical model combining several regulatory cycles from the budding yeast cell cycle. IET systems biology 1, 326-341. Steuer, R. (2004). Effects of stochasticity in models of the cell cycle: from quantized cycle times to noise-induced oscillations. Journal of Theoretical Biology 228, 293301. Tigges, M., Marquez-Lago, T.T., Stelling, J., and Fussenegger, M. (2009). A tunable synthetic mammalian oscillator. Nature 457, 309-312. Tsai, T.Y.-C., Choi, Y.S., Ma, W., Pomerening, J.R., Tang, C., and Ferrell, J.E. (2008). Robust, tunable biological oscillations from interlinked positive and negative feedback loops. Science 321, 126-129. Tsai, T.Y.C., Theriot, J.A., and Ferrell, J.E., Jr. (2014). Changes in Oscillatory Dynamics in the Cell Cycle of Early Xenopus laevis Embryos. PLoS Biol 12, e1001788. Tyson, J.J. (1991). Modeling the cell division cycle: cdc2 and cyclin interactions. Proceedings of the National Academy of Sciences 88, 7328-7332. Tyson, J.J., Albert, R., Goldbeter, A., Ruoff, P., and Sible, J. (2008). Biological switches and clocks. Journal of The Royal Society Interface 5, S1-S8. Tyson, J.J., Hong, C.I., Dennis Thron, C., and Novak, B. (1999). A simple model of circadian rhythms based on dimerization and proteolysis of PER and TIM. Biophysical journal 77, 2411-2417. Zámborszky, J., Hong, C.I., and Csikász-Nagy, A. (2007). Computational analysis of mammalian cell division gated by a circadian clock: quantized cell cycles and cell size control. Journal of biological rhythms 22, 542-553.



What makes a biological clock efficient? - Microsoft

What makes a biological clock efficient? Attila Csikász-Nagy1,2, Neil Dalchau3 1 Randall Division of Cell and Molecular Biophysics and Institute for ...

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